Inadequate transportation infrastructure is a pressing challenge in rapidly expanding urban centers globally. However, the differential impacts of infrastructure improvements for women versus men remain inadequately understood and underexplored. We address this knowledge gap by documenting gender-specific travel patterns and perceptions of safety in Dhaka, Bangladesh. Specifically, we analyze how the introduction of Dhaka’s first mass rapid transit (MRT) line has altered modes of transportation, the frequency of travel, and safety-related concerns among women. Our empirical findings highlight that the new MRT line has notably influenced women’s travel behaviors, particularly by alleviating safety concerns that previously discouraged regular travel.